Monday, March 4, 2019
Enzyme Lab
Purpose and BackgroundThe purpose of this lab is to explain how enzymes act as catalysts for biological reactions in dissimilar temperatures. This lab relates to enzymes, proteins, and substrates that we learned in class. The conjugation of the enzyme and the substrate is called the enzyme-substrate complex. The make-up of an enzyme is proteins and made up of chains and amino acids.Enzymes ar considered to be organic catalysts, they speed up chemical reactions that might other take too long to occur and be of use in the body. They to a fault allow reactions to occur in regions of the body that are unfavorable because of the level of pH or the lack of heat. HypothesisA reused and ground enzyme will comport the greatest reactions compared to the other types of enzymes. Materials 1. Avocado 2.Petri Dish 3.Hydrogen Peroxide 4.Vinegar 5.Vinegar SubstrateProcedure1.Place a dinky piece of cooked alligator pear/avocado/soaked avocado in a Petri dish. 2.Place 10 drops of atomic number 1 peroxide/ vinegar substrate on the avocado 3.Observe for enzyme activity by looking for bubbles4.Rate the amount of oxygen bubbles produced on a scale of 1-5 5.Record your data in the chartDataConclusionAfter placing 10 drops of hydrogen peroxide and vinegar substrate in the different forms of avocado, the results were interesting. The ground and reused enzyme had the most evident reactions. It also shows me that enzymes can be reused, because of the reaction rating. Enzymes can be affected by temperatures because the regular enzyme reaction is low. Also enzymes arent affected by pH because it had a low reaction. Enzymes are specific because different types of enzymes work for different substrates.DiscussionMy hypothesis was supported and proved in the lab. I predicted that the ground and reused enzyme would discombobulate the greatest reaction rating, and they did. Evidence that occurred in the lab would be the hydrogen peroxide and vinegar substrate dropped onto the different forms of enzymes. I dont think any improvements can be made, the lab was straight prior and proved what needed to be known
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